Identification of an in Vivo Inhibitor of Bacillus anthracis Spore Germination

Identification of an in Vivo Inhibitor of Bacillus Anthracis Sterne Spore Germination

*Correspondence should be addressed to Ernesto Abel-Santos or Jürgen Brojatsch. EAS: Department of Chemistry, University of Nevada Las Vegas, 4505 Maryland Pkwy-Box 454003, Las Vegas, NV 89154, Tel. 702 895-2608, Fax. 702 895-4072, E-mail: [email protected]. JB: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Golding 404, 1300 Morris Park Avenue, Bronx,...

Identification of an in vivo inhibitor of Bacillus anthracis spore germination.

Germination of Bacillus anthracis spores into the vegetative form is an essential step in anthrax pathogenicity. This process can be triggered in vitro by the common germinants inosine and alanine. Kinetic analysis of B. anthracis spore germination revealed synergy and a sequential mechanism between inosine and alanine binding to their cognate receptors. Because inosine is a critical germinant ...

Detection of Bacillus anthracis spore germination in vivo by bioluminescence imaging.

We sought to visualize the site of Bacillus anthracis spore germination in vivo. For that purpose, we constructed a reporter plasmid with the lux operon under control of the spore small acid-soluble protein B (sspB) promoter. In B. subtilis, sspB-driven synthesis of luciferase during sporulation results in incorporation of the enzyme in spores. We observed that B. anthracis Sterne transformed w...

Spore Germination and Outgrowth Spore Detection and Functional Analyses of Bacillus anthracis Monoclonal Antibodies for

Proteomic profiling and identification of immunodominant spore antigens of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis.

Differentially expressed and immunogenic spore proteins of the Bacillus cereus group of bacteria, which includes Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis, were identified. Comparative proteomic profiling of their spore proteins distinguished the three species from each other as well as the virulent from the avirulent strains. A total of 458 proteins encoded by 232 open re...